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1 High Resolution Thin-Film Thermistors . . . . . . . . 2 Miniaturized Enzyme Thermistors . . . . . . . . . . 3 Integrated Thermopiles . . . . . . . . . . . . . 62 Advances in Biochemical Engineering / Biotechnology, Vol. 64 Managing Editor: Th. Scheper © Springer-Verlag Berlin Heidelberg 1999 36 F. Lammers · Th. 4 Bio-Thermochips . . . . . . . . . . . . . . . 5 Compact Multichannel Enzyme Thermistors . . . . . . . 63 6 Conclusions .

Immobilized a-chymotrypsin hydrolyzes the artificial sweetener under release of protons which are easily detected via a tris/HCl-buffer due to its high protonation enthalpy. The immobilized enzyme unfortunately causes an unspecific conversion. However, the procedure might be interesting for monitoring aspartame during its industrial production within the Tosoh-process. 60 F. Lammers · Th. Scheper 250 200 100 units catalase peak area [V*s] 150 100 1000 units catalase 50 0 0,0 addition of H2O2 2,5 5,0 new addition of H2O2 7,5 10,0 12,5 15,0 time [h] Fig.

The latter describes the ratio of amplified and non-amplified signal in the linear 50 F. Lammers · Th. Scheper Fig. 8. Amplification system of lactate oxidase (LOD), lactate dehydrogenase (LDH) and catalase (CAT) range. Kirstein et al. (1989) describe an ET analyzing ATP with a detection limit of 10 nmol/l. The enzymatic cycling system causes an amplifiction factor about 1700. Another amplification system uses a combination of lactate oxidase, lactate dehydrogenase and catalase (LOD/LDH/CAT-system; Fig.

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